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Category: Carbohydrate analysis

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What is the difference between Barfoed's test and Benedict's test?

4 min read
Barfoed's test distinguishes monosaccharides from disaccharides, whereas Benedict's test is a general test for all reducing sugars. This crucial difference between Barfoed's test and Benedict's test stems from the varying pH of their reagents, with Barfoed's operating in acidic conditions and Benedict's in an alkaline medium.