Tag: Absorbance

The average adult human body contains 4–6 grams of iron, making its accurate measurement a critical task in many fields. The maximum absorbance of iron is not a fixed value for the element itself but depends on the specific chemical form, known as a complex, into which it is converted for spectrophotometric analysis. This process is vital for applications ranging from environmental monitoring to pharmaceutical quality control.

The common food coloring Red 40, also known as Allura Red AC, exhibits its maximum absorbance in the green-blue region of the visible light spectrum. This characteristic property is critical for both its use in food manufacturing and its analysis in a laboratory through techniques like spectrophotometry.

Reduced glutathione (GSH) possesses no significant intrinsic absorbance in the standard ultraviolet (UV) or visible spectrum, necessitating a chemical reaction for spectrophotometric quantification. Instead of a direct measurement, its concentration is determined by reacting its thiol group with chromogenic reagents to produce a colored compound with a measurable absorbance.