Tag: Beer lambert law

The average adult human body contains 4–6 grams of iron, making its accurate measurement a critical task in many fields. The maximum absorbance of iron is not a fixed value for the element itself but depends on the specific chemical form, known as a complex, into which it is converted for spectrophotometric analysis. This process is vital for applications ranging from environmental monitoring to pharmaceutical quality control.

According to a study comparing various methods for measuring carotenoids, UV-Vis spectrophotometry provides a highly convenient way to determine total carotenoid concentration. Calculating beta-carotene content, however, requires careful technique, from proper sample extraction to applying a standard curve or advanced chromatography.

The common food coloring Red 40, also known as Allura Red AC, exhibits its maximum absorbance in the green-blue region of the visible light spectrum. This characteristic property is critical for both its use in food manufacturing and its analysis in a laboratory through techniques like spectrophotometry.