Tag: Blocking buffer

In many biological assays, such as Western blotting and ELISA, blocking non-specific binding sites is a critical step to ensure accurate results. Properly learning how to make up 1% BSA solution is a fundamental laboratory skill that facilitates this process, providing a stable, reliable blocking agent that minimizes background noise.

While the term 'nutrition diet' points toward a dietary context, the phrase '**why is milk a good blocking buffer**' refers to a key step in molecular biology research, specifically Western blotting. In laboratories worldwide, non-fat dry milk is a common, inexpensive reagent used to saturate membranes, preventing antibodies from binding non-specifically and causing background noise. This surprising application is a cornerstone of immunodetection techniques, demonstrating milk's critical utility beyond the kitchen.