Tag: Cell viability

Scientific studies established in the mid-20th century that supplementing media with vitamins is essential for the growth and proliferation of cells in vitro. Understanding what is the role of vitamins in cell culture is therefore foundational for maintaining healthy and reproducible cellular experiments.

Originally developed in the early 1980s, the N2 supplement revolutionized neural cell culture by providing a chemically defined, serum-free alternative to traditional fetal bovine serum. The specific components of N2 supplement are instrumental for ensuring the viability, growth, and proper differentiation of neuronal cells, minimizing experimental variability and supporting highly specialized neurobiological studies.

B-27 supplement is a serum-free formulation widely used in neuroscience research for over 25 years to promote the survival and maturation of cultured neurons. This complex mixture of vitamins, antioxidants, fatty acids, and other key components provides a defined environment for neural cells in vitro.

According to biomedical researchers, cells are incredibly sensitive to their surrounding environment, requiring a precisely controlled balance of salts and electrolytes to survive. In the laboratory, the question of what is the best salt for cells is critical for ensuring experimental accuracy and maintaining cell viability. This article explores the science behind balanced salt solutions and their importance for cellular function.