Tag: Chromatography

According to the Food and Drug Administration (FDA), food fraud is estimated to cost the global food industry around $10 to $15 billion annually. Identifying sophisticated food fraud requires highly advanced analytical techniques to pinpoint the exact nature and origin of undeclared substances. But what is the most precise method used to identify adulteration in food? While a single 'most precise' method depends on the adulterant, techniques combining chromatography and mass spectrometry (GC-MS/LC-MS) are universally recognized for their unparalleled accuracy and sensitivity.

Trace metal ions can significantly interfere with downstream protein analysis by promoting aggregation or inhibiting enzymes. Understanding how to remove metal from protein is a critical step in achieving high-purity and functional results.

Polysaccharides, complex carbohydrate polymers like starch and cellulose, are ubiquitous in nature and play crucial roles in living organisms. To determine a polysaccharide in a sample, a variety of analytical techniques, from simple chemical reactions to advanced instrumental methods, are required to reveal its structural and compositional properties.

Over 50% of the fatty acid composition in common cooking oils like safflower and corn is C18:2, also known as linoleic acid. However, the term "C18 2" also refers to a specific type of C18 column used in analytical chemistry. Context is key to understanding what is C18 2.

Over 1.6 million different lipid species have been identified, and their complex and diverse structures present a formidable challenge for comprehensive analysis. A typical lipid analysis workflow involves sequential steps, including sample preparation, lipid extraction, separation, and detection, to accurately define and quantify the lipid composition of a sample.

The worldwide production of sugar exceeded 185 million tonnes in 2017, highlighting the crucial need for accurate methods of sucrose determination in the food industry and beyond. A range of analytical techniques exists, from simple physical measurements to highly specific and sensitive enzymatic assays, each suited for particular applications and sample types.

According to research, the production of protein concentrates and isolates has evolved significantly, moving beyond traditional sources like soybeans to encompass a wider variety of food sources through advanced processing. The concentration of protein is a crucial step in the production of supplements and functional foods, with several key methods used depending on the desired outcome.

Carbohydrates are the most abundant and diverse class of organic compounds in nature, serving as a primary energy source in diets. A wide range of analytical techniques are used to identify and quantify these vital macromolecules in various samples, from food to biological fluids.

According to a 2018 review in *IntechOpen*, thousands of individual lipid molecular species exist in cells, making comprehensive analysis challenging. A range of advanced analytical tools, from mass spectrometry to nuclear magnetic resonance, helps to identify the lipid structure with high precision and accuracy by revealing details about fatty acyl chains, headgroups, and stereochemistry.

According to the LIPID MAPS consortium, there are over 43,000 structurally distinct lipids registered, highlighting their immense diversity and the complexity involved in their analysis. To determine the lipid structure, scientists rely on a combination of powerful analytical techniques that provide detailed molecular information, from mass composition to spatial arrangement. This process is crucial for understanding the biological functions and health implications of these essential biomolecules.