Tag: Colorimetric assay

Phytase activity is a critical metric in animal nutrition, with one phytase unit (FTU) officially defined as the amount of enzyme that liberates 1 micromole of inorganic phosphorus per minute. The formula for phytase activity depends on the specific assay used, but fundamentally quantifies the rate of inorganic phosphate release from a phytate substrate under controlled laboratory conditions.

The Biuret test is a widely used laboratory technique for detecting the presence of proteins, and its success is most notably defined by a distinctive color change. This colorimetric reaction produces a violet result, which mystifies many, but the answer to what is the violet color of the Biuret test lies in a specific chemical coordination reaction that indicates the presence of proteins.

The color change in a Biuret test is a key indicator for the presence of protein. A light blue color indicates a negative test for protein, confirming that the sample lacks a significant concentration of peptide bonds. This simple colorimetric test is a cornerstone of basic biochemistry labs for detecting proteins.

According to the scientific literature, lipids are a vast and diverse group of molecules, constituting a formidable challenge for comprehensive analysis due to their structural complexity. Quantifying these molecules is essential in life sciences and clinical diagnostics, driving the development of numerous analytical techniques to determine exactly how you quantify lipids in various samples.