How much protein for dot blot: A comprehensive guide
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4 min read
While Western blotting requires loading 10-50 µg of protein per lane for cell lysates, dot blotting can require significantly less, sometimes as little as 0.5-1 µg per spot. The amount of protein to use for a dot blot is highly dependent on the sample type, the abundance of the target protein, and the sensitivity of the antibodies being used.
