What is the Absorbance of Reduced Glutathione?
•
4 min read
Reduced glutathione (GSH) possesses no significant intrinsic absorbance in the standard ultraviolet (UV) or visible spectrum, necessitating a chemical reaction for spectrophotometric quantification. Instead of a direct measurement, its concentration is determined by reacting its thiol group with chromogenic reagents to produce a colored compound with a measurable absorbance.