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Tag: Dtnb

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What is the Absorbance of Reduced Glutathione?

4 min read
Reduced glutathione (GSH) possesses no significant intrinsic absorbance in the standard ultraviolet (UV) or visible spectrum, necessitating a chemical reaction for spectrophotometric quantification. Instead of a direct measurement, its concentration is determined by reacting its thiol group with chromogenic reagents to produce a colored compound with a measurable absorbance.