Tag: Immunodetection

While Western blotting requires loading 10-50 µg of protein per lane for cell lysates, dot blotting can require significantly less, sometimes as little as 0.5-1 µg per spot. The amount of protein to use for a dot blot is highly dependent on the sample type, the abundance of the target protein, and the sensitivity of the antibodies being used.

While the term 'nutrition diet' points toward a dietary context, the phrase '**why is milk a good blocking buffer**' refers to a key step in molecular biology research, specifically Western blotting. In laboratories worldwide, non-fat dry milk is a common, inexpensive reagent used to saturate membranes, preventing antibodies from binding non-specifically and causing background noise. This surprising application is a cornerstone of immunodetection techniques, demonstrating milk's critical utility beyond the kitchen.