Tag: Laboratory protocol

According to scientific protocols, biotin phenol is often supplied as a solid, and its successful dissolution is a critical first step in applications like proximity labeling. For researchers needing to prepare working solutions, understanding the proper solvents and techniques to dissolve biotin phenol is essential for accurate and consistent experimental results.

DCPIP (2,6-dichlorophenolindophenol) is a redox dye that is blue in its oxidized state and colorless when reduced, making it a valuable tool in biochemistry. Accurately preparing this solution is critical for experiments like vitamin C titration or studying photosynthesis. Learn exactly how to prepare DCPIP in the laboratory with this detailed and reliable protocol.

While the term 'nutrition diet' points toward a dietary context, the phrase '**why is milk a good blocking buffer**' refers to a key step in molecular biology research, specifically Western blotting. In laboratories worldwide, non-fat dry milk is a common, inexpensive reagent used to saturate membranes, preventing antibodies from binding non-specifically and causing background noise. This surprising application is a cornerstone of immunodetection techniques, demonstrating milk's critical utility beyond the kitchen.