Tag: Redox

Vitamin C is a powerful antioxidant and scavenger of free radicals in biological systems. Its primary function involves neutralizing harmful Reactive Oxygen Species (ROS) that are constantly produced during normal metabolic processes and from environmental stressors like pollution and radiation. This crucial process helps protect cells from damage and reduces oxidative stress.

Reduced glutathione (GSH) possesses no significant intrinsic absorbance in the standard ultraviolet (UV) or visible spectrum, necessitating a chemical reaction for spectrophotometric quantification. Instead of a direct measurement, its concentration is determined by reacting its thiol group with chromogenic reagents to produce a colored compound with a measurable absorbance.