Does LB Broth Need to Be Refrigerated? A Guide for Lab Storage
                                
                                
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                                    4 min read                                
                            
                                According to laboratory protocols, storing sterile, prepared LB broth at cold temperatures significantly extends its viability and prevents contamination. This is because LB broth is a nutrient-rich medium designed to promote bacterial growth, and leaving it at room temperature will compromise its sterility and suitability for experiments.