Tag: Seliwanoff test

The Seliwanoff test is a biochemical test that uses a timed color reaction to distinguish between ketose and aldose sugars. Specifically, what does Seliwanoff test for and what chemical principles allow it to differentiate between these two classes of carbohydrates based on their functional groups?

The Seliwanoff test, a common method for distinguishing ketoses from aldoses, relies on a chemical reaction involving concentrated hydrochloric acid and resorcinol. Due to the use of these hazardous substances and a heating step, stringent precautions are necessary to protect laboratory personnel and guarantee accurate results.

The Seliwanoff's test is a foundational biochemical assay that distinguishes between aldose and ketose sugars, a distinction crucial for carbohydrate analysis. A positive Seliwanoff test, indicated by a deep cherry-red color, confirms the presence of a ketose or a sugar that can be hydrolyzed into one.