Tag: Serum free media

According to scientific literature, fetal bovine serum (FBS) is an exceptionally complex mixture containing over 1,000 components, including amino acids like arginine. This critical component plays a multifaceted role in supporting cell growth, proliferation, and metabolism in various in vitro systems.

Defined, serum-free media are now the standard in cell culture, consistently offering greater reproducibility than serum-based alternatives. The N2 supplement, a foundational medium in neurobiology, contains five core components that provide the necessary support for neural cells to thrive and differentiate in vitro.

Over 11,000 scientific publications cite the use of the B27 supplement, a specialized medium additive used exclusively in laboratory settings for growing and maintaining neuronal cells. It is a common misconception that 'B27 supplement' is for human health, but its function is confined to biomedical research, specifically for cell culture applications.

Originally developed in the early 1980s, the N2 supplement revolutionized neural cell culture by providing a chemically defined, serum-free alternative to traditional fetal bovine serum. The specific components of N2 supplement are instrumental for ensuring the viability, growth, and proper differentiation of neuronal cells, minimizing experimental variability and supporting highly specialized neurobiological studies.

B-27 supplement is a serum-free formulation widely used in neuroscience research for over 25 years to promote the survival and maturation of cultured neurons. This complex mixture of vitamins, antioxidants, fatty acids, and other key components provides a defined environment for neural cells in vitro.

In neuroscience, over 90% of cells in a healthy brain are neurons or glial cells, underscoring the need for specialized media to study them effectively. This is where the N2 supplement becomes critical, as it is a chemically defined, serum-free additive designed specifically to support the growth and survival of neuronal cell types in vitro.