Tag: Standard curve

According to a study comparing various methods for measuring carotenoids, UV-Vis spectrophotometry provides a highly convenient way to determine total carotenoid concentration. Calculating beta-carotene content, however, requires careful technique, from proper sample extraction to applying a standard curve or advanced chromatography.

Over 90% of all protein quantification assays rely on colorimetric methods to determine concentration. Learning how to do protein estimation accurately is a foundational skill in biology and biochemistry, essential for a wide range of experiments from preparing samples for electrophoresis to enzyme activity studies. This guide will break down the most reliable techniques for estimating protein concentration and provide practical steps for success in your laboratory.