Tag: Western blotting

In many biological assays, such as Western blotting and ELISA, blocking non-specific binding sites is a critical step to ensure accurate results. Properly learning how to make up 1% BSA solution is a fundamental laboratory skill that facilitates this process, providing a stable, reliable blocking agent that minimizes background noise.

Over 99% of biological research labs utilize protein gel electrophoresis to analyze protein samples. A protein gel, typically made of polyacrylamide, acts as a molecular sieve to separate complex mixtures of proteins based on their physical properties, enabling scientists to study them individually. By applying an electric current, proteins are sorted by size, charge, or both, providing crucial insights into their characteristics, function, and abundance.