The Science of Vitamin K2 Production in Fermented Foods
Vitamin K2 is a collection of compounds known as menaquinones (MKs), which are produced by certain types of bacteria during fermentation. In the context of dairy, bacteria used in mesophilic fermentation (room temperature) are particularly effective at producing K2, while thermophilic cultures (high heat, like many yogurts) produce very little or none. The kefir grain cultures, with their diverse and robust bacterial strains and yeasts, are known to be efficient at this process, creating various menaquinone forms like MK-7, MK-8, and MK-9. The specific menaquinone profile and quantity can vary widely depending on the starter culture, fermentation conditions, and length of the process. A 2017 study found that low-fat kefir contained 10.2 µg total vitamin K per 100g, while another 2019 study on Finnish kefir found lower levels, demonstrating this variability.
The Relationship Between Fat Content and K2
Vitamin K is a fat-soluble vitamin, which means it dissolves in fat and is absorbed most efficiently when consumed with dietary fat. Because of this, full-fat dairy products generally contain higher concentrations of K2 than their low-fat or skimmed counterparts. When milk is processed to become low-fat kefir, much of the milk fat, which is where the K2 would be concentrated, is removed. While the probiotic activity in low-fat kefir can still produce some K2, the final amount is significantly lower compared to a full-fat version. Some researchers even note that while some probiotic activity may produce K2, the fat content is the primary factor affecting the final concentration in the dairy product. This means that anyone seeking to maximize their K2 intake from kefir should opt for the full-fat variety.
How Fermentation Time and Culture Affects K2 Levels
The fermentation process is a dynamic one, and the resulting vitamin content is a direct reflection of the microbial activity. Studies have shown that K2 levels increase over the duration of fermentation as the bacterial population multiplies and works to produce menaquinones.
- Longer fermentation: A longer fermentation period can lead to higher levels of K2, as the bacteria have more time to synthesize the compound. For example, a 15-hour kefir grain fermentation in a 2017 study was shown to have significantly higher MK-7 content than milk fermented for a shorter period.
- Starter Culture: The type of bacteria in the kefir grains is critical. Different strains produce different menaquinones, some in higher quantities than others. Some commercial starter cultures may yield different results compared to traditional, live kefir grains. The most potent K2 sources, such as Japanese natto, use specific strains of Bacillus subtilis.
Other Good Sources of Vitamin K2
To increase your dietary intake of Vitamin K2, consider other food sources, particularly those that are fermented or rich in fat.
- Natto: This Japanese fermented soybean dish is the single richest source of K2 (specifically MK-7), with significantly higher amounts than even full-fat dairy.
- Hard and Soft Cheeses: Cheeses made with mesophilic cultures, such as Gouda and Brie, are excellent sources of longer-chain menaquinones like MK-8 and MK-9.
- Animal Products: Animal products from grass-fed sources tend to have higher levels of MK-4. These include butter, eggs, and liver.
- Sauerkraut: Some fermented vegetables contain K2 due to bacterial activity, though typically in lower concentrations than fermented dairy.
Comparison of K2 Content in Kefir and Other Foods
| Food Type | K2 Content (µg/100g) | Notes |
|---|---|---|
| Full-Fat Kefir | Up to 13 µg | Variable, dependent on culture and fat content. |
| Low-Fat Kefir | Less than Full-Fat | Substantially lower; a 2017 study found ~10 µg total K, including other K vitamins. |
| Hard Cheese | 30-80 µg | Good source of MK-8 and MK-9 from bacteria. |
| Natto | >1000 µg | Exceptionally high concentration of MK-7. |
| Chicken Breast | 13 µg per 85g | Contains MK-4, though content can be lower. |
| Grass-Fed Butter | 13-21 µg | Source of MK-4, with levels depending on diet. |
Conclusion: Making an Informed Choice
So, does lowfat kefir have K2? The definitive answer is yes, but in a significantly lower quantity than full-fat kefir and other rich dietary sources like specific cheeses and natto. The production of Vitamin K2 is a function of bacterial fermentation, but its fat-soluble nature means it is primarily retained within the milk fat. For those looking to increase their Vitamin K2 intake, relying solely on lowfat kefir would be inefficient. A balanced approach would involve incorporating a variety of K2-rich foods into the diet, including full-fat fermented dairy, certain cheeses, and potentially natto, while also supporting overall gut health with probiotics. Consulting with a healthcare provider can help determine the best dietary strategy for individual needs, especially regarding the synergistic effects of vitamins D3 and K2. A balanced diet focusing on whole, fermented foods provides the best approach to ensuring adequate levels of this crucial nutrient for bone and cardiovascular health.
Evaluation of MENAQUINONE-7 and fat-soluble vitamin content...