Skip to content

Does Mustard Powder Contain Myrosinase? An In-Depth Look

3 min read

A 2018 study on human subjects demonstrated that adding just one gram of brown mustard powder to cooked broccoli increased the bioavailability of the health-promoting compound sulforaphane by over four times. This potent effect occurs because mustard powder contains myrosinase, the very enzyme needed for this reaction.

Quick Summary

Mustard powder does contain the active enzyme myrosinase, which is crucial for converting glucosinolates into beneficial isothiocyanates. This enzyme, naturally present in mustard seeds, survives the drying and grinding process, remaining potent and ready to be activated with water.

Key Points

  • Yes, it contains active myrosinase: Mustard powder retains the myrosinase enzyme from the original seeds, which is activated when mixed with water.

  • Enhances cooked veggies: Adding mustard powder to cooked cruciferous vegetables like broccoli can re-supply the heat-destroyed myrosinase and increase sulforaphane bioavailability.

  • Distinct from prepared mustard: The heat and acid used in making most prepared mustards completely denature the myrosinase enzyme, rendering it inactive.

  • Activation requires water: The chemical reaction between myrosinase and glucosinolates is triggered by the addition of cold or lukewarm water to the powder.

  • Timing is key: For maximum enzymatic activity, mustard powder should be added to food after cooking, as high temperatures will deactivate the enzyme.

  • More resilient enzyme: The myrosinase in mustard is more resistant to heat than the enzyme in other cruciferous vegetables, making it an excellent dietary additive.

In This Article

Yes, Mustard Powder Contains Active Myrosinase

Contrary to some assumptions about powdered products, mustard powder does contain active myrosinase. Myrosinase is a class of enzymes found in plants of the Brassica family, which includes mustard, broccoli, and cabbage. The enzyme is stored in separate compartments from its substrates, compounds called glucosinolates. When the plant tissue is damaged through crushing, chewing, or grinding, these two components mix, triggering a chemical reaction. This process is the foundation of mustard's characteristic pungent heat and many of its health benefits.

The Resilient Nature of Mustard Myrosinase

While many enzymes are sensitive to heat and denature easily, the myrosinase found in mustard seeds is particularly resilient. This is why adding mustard powder to cooked foods is so effective. For example, cooking broccoli at high temperatures destroys its natural myrosinase, preventing the formation of sulforaphane from its precursor, glucoraphanin. However, by sprinkling active mustard powder onto the cooked broccoli, you reintroduce the necessary enzyme to complete this process and unlock the vegetable's full potential.

How the Enzyme Works in Dry Powder

In its dry, powdered state, the myrosinase enzyme is inactive. This is because the chemical reaction with glucosinolates requires water. When you mix mustard powder with a cold liquid, such as water or vinegar, the enzyme becomes active, and the hydrolysis reaction begins. Hot water or high heat will deactivate the enzyme, so for maximum effect, the powder should be mixed with cold or lukewarm water and added to dishes just before serving.

Myrosinase Activity: Mustard Powder vs. Prepared Mustard

Feature Mustard Powder Prepared Mustard (e.g., Dijon, Yellow)
Myrosinase Activity Active. The grinding and drying process does not typically destroy the enzyme. It is activated with water. Inactive. Most prepared mustards are processed with heat and an acidic liquid (like vinegar), which denatures and destroys the enzyme.
Processing Simply dried and ground mustard seeds. Seeds are ground and mixed with liquid, spices, and often heated to create the final product.
Flavor Profile Intense and pungent, depending on the mustard seed type. The heat is released upon activation with water. Varies widely based on ingredients. Heat is typically less intense and more stable due to enzyme inactivation.
Usage Use to activate isothiocyanates in cooked foods or to create custom condiments. Primarily used as a flavorful condiment; provides no myrosinase activity for external foods.

Optimizing the Benefits of Mustard Powder

To get the most out of your mustard powder and its myrosinase content, follow these simple steps:

  1. Select a High-Quality Powder: Choose a reputable brand or grind your own fresh mustard seeds. The “bite” of the powder when mixed with cold water is a good indicator of active myrosinase.
  2. Use Cold Water: When preparing a sauce or activating the powder for cooking, use cold or lukewarm water. This preserves the myrosinase before adding it to other foods.
  3. Add After Cooking: To enhance the health benefits of other vegetables, like cooked broccoli, cauliflower, or cabbage, add the activated mustard powder after the cooking process is complete. The high heat of cooking would otherwise render the enzyme ineffective.
  4. Allow Time to React: After mixing the powder with water, give it a few minutes to react before adding it to your food. This ensures the maximum formation of beneficial compounds.

The Health Significance of Myrosinase Activity

The conversion of glucosinolates to isothiocyanates by myrosinase is a powerful mechanism for producing health-beneficial compounds. Research has linked isothiocyanates, such as sulforaphane, to various protective effects in the body, including potential anti-cancer properties and antioxidant benefits. By understanding that mustard powder contains myrosinase and how to properly utilize it, you can intentionally enhance the nutritional value of many meals, especially those containing other cruciferous vegetables.

For more in-depth information on the chemical process, consider reviewing publications such as the study on exogenous myrosinase and sulforaphane bioavailability.

Conclusion

In conclusion, yes, mustard powder does contain active myrosinase, an enzyme vital for producing pungent flavors and health-enhancing compounds. Unlike prepared mustards, which inactivate the enzyme through heat and acidity, dry mustard powder retains its enzymatic activity, ready to be unleashed upon contact with water. By properly incorporating mustard powder into your cooking, particularly with other cooked cruciferous vegetables, you can leverage this natural enzymatic powerhouse to unlock significant nutritional benefits. This simple culinary trick is a testament to the complex biochemistry found within everyday food and provides a straightforward way to boost your diet's protective properties.

Frequently Asked Questions

Yes, as long as it hasn't been exposed to high heat or prolonged acidic conditions, the myrosinase enzyme in mustard powder remains active and can be triggered by adding cold water.

Cooking broccoli destroys its naturally occurring myrosinase enzyme. Adding mustard powder provides an external, active source of myrosinase, which can then convert the broccoli's glucosinolates into health-beneficial sulforaphane.

No. Most prepared mustards are made with heat and vinegar, both of which denature and inactivate the myrosinase enzyme. Therefore, prepared mustard cannot be used to activate beneficial compounds in other foods.

The myrosinase enzyme found in mustard seeds is known to be more thermally stable and resilient to heat than the myrosinase in vegetables like broccoli, which is why it's so useful as a dietary additive.

Myrosinase activity can diminish over time, especially if exposed to moisture. For maximum potency, it is best to use fresh mustard powder and store it in a cool, dry place.

In plants, the myrosinase-glucosinolate system acts as a defense mechanism against herbivores and pathogens. The reaction produces toxic isothiocyanates when the plant is damaged.

Yes. When mixed with cold water, active mustard powder should develop a pungent, sharp flavor due to the chemical reaction creating isothiocyanates. If it doesn't, the enzyme may be inactive.

References

  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

Medical Disclaimer

This content is for informational purposes only and should not replace professional medical advice.