Tag: Lowry method

The total protein content of biological samples is a critical parameter in numerous research and clinical applications. Determining the amount of protein requires a reliable quantitative test for proteins, with several established methods available, each with unique characteristics and optimal use cases.

According to Thermo Fisher Scientific, quantifying protein concentration is an essential part of most laboratory workflows involving protein samples. The method for determining total protein varies based on required sensitivity, sample volume, and compatibility with other substances.

Over 90% of all protein quantification assays rely on colorimetric methods to determine concentration. Learning how to do protein estimation accurately is a foundational skill in biology and biochemistry, essential for a wide range of experiments from preparing samples for electrophoresis to enzyme activity studies. This guide will break down the most reliable techniques for estimating protein concentration and provide practical steps for success in your laboratory.