Skip to content

Are Probiotics Temperature Sensitive? The Definitive Guide to Storage

4 min read

Over 1.4 million American adults used probiotics in 2021, and they are generally sensitive to changes in temperature and humidity. The viability and effectiveness of these living microorganisms depend heavily on their storage conditions, which is a crucial factor to consider when purchasing and using probiotic supplements.

Quick Summary

Probiotic organisms are sensitive to temperature and humidity, which impacts their viability and effectiveness. High heat kills beneficial bacteria, while refrigeration or advanced packaging can preserve them. The proper storage method depends on the specific product and its formulation.

Key Points

  • Sensitivity to Temperature: Probiotics are living organisms highly sensitive to temperature fluctuations and humidity; high heat can kill them, reducing effectiveness.

  • Heat Kills Bacteria: Temperatures over 120°F (49°C) cause probiotic cells to die rapidly due to protein denaturation and cell structure disruption.

  • Refrigeration vs. Shelf-Stable: Some probiotics require refrigeration to maintain viability, while others are freeze-dried and packaged to be shelf-stable at room temperature.

  • Read the Label: Always check the product's label for specific storage instructions, as manufacturer guidelines vary based on the strains and formulation.

  • Proper Handling is Key: Store probiotics in a cool, dry place away from sunlight, use original packaging, and avoid consuming with hot beverages to protect their viability.

  • Shelf-Stable Convenience: Modern packaging and freeze-drying technology make many probiotics convenient for travel, offering an alternative to refrigerated options.

In This Article

Understanding Why Probiotics Are Temperature Sensitive

Probiotics are living, beneficial microorganisms, and their core function is dependent on being alive when they reach your gut. As with any living organism, they have specific environmental needs to survive and thrive. Temperature is one of the most critical factors influencing their stability and lifespan.

The Impact of High Temperatures on Probiotics

When exposed to high heat, such as temperatures exceeding 120°F (49°C), probiotic cultures begin to die off rapidly. This happens because high temperatures cause the denaturation of proteins and the disruption of the delicate cellular structures of the bacteria. This is why fermented foods like kimchi and sauerkraut, marketed for their live cultures, should not be exposed to high heat during cooking. For supplements, leaving a bottle in a hot car or near a heat source can drastically reduce the number of live colony-forming units (CFUs) by the time they are consumed.

The Role of Cold Temperatures and Moisture

Conversely, cold temperatures, like those in a refrigerator, help to prolong the potency and viability of most probiotics. Refrigeration provides a stable, low-humidity environment that keeps the bacteria in a dormant state, preventing them from becoming active and dying off prematurely. This is why many traditional probiotic supplements and food products, such as yogurt and kefir, require refrigeration. Moisture is another enemy of probiotic stability, and refrigeration helps to mitigate this risk as well.

The Evolution of Probiotic Technology: Shelf-Stable vs. Refrigerated

Not all probiotics are created equal, and advances in technology have led to the development of different storage requirements for various products. The need for refrigeration is not a universal rule.

Shelf-Stable Probiotics

  • Freeze-Drying (Lyophilization): Many modern probiotics are made shelf-stable through a freeze-drying process. This method removes moisture, putting the bacteria into a dormant, spore-like state that protects them from heat and moisture damage. Once consumed and re-exposed to moisture in the body, they can reactivate. Bacillus strains are a common example of heat-tolerant probiotics used in shelf-stable products.
  • Advanced Packaging: Manufacturers also use specialized packaging technologies, such as moisture-resistant sachets or unique activ-vial bottles, to create a protective barrier against environmental factors. This allows the product to remain viable for up to two years at room temperature.

Refrigerated Probiotics

  • Live Cultures: Traditional probiotics in foods like yogurt, kefir, and certain supplement strains (like some Lactobacillus and Bifidobacterium species) are too sensitive to survive outside of a refrigerated environment. Refrigeration keeps these delicate live cultures alive and at full potency until their expiration date.

Understanding Probiotic Packaging and Labeling

Knowing how your specific probiotic product was processed and packaged is key to ensuring its efficacy. Always follow the storage instructions on the label.

  • “Refrigerate” or “Store at 2°C–8°C”: These labels indicate that the product contains live, delicate cultures that require cold storage to maintain viability.
  • “Store Below 25°C” or “No Refrigeration Required”: These products are typically shelf-stable due to freeze-drying or advanced packaging. They should still be stored in a cool, dry place away from direct sunlight.
  • CFU Count: Check the label for the CFU count and whether it's guaranteed at the time of manufacture or through the expiration date. A CFU count guaranteed through the expiration date is a better indicator of product quality.

How to Handle and Store Probiotics Correctly

To get the most benefit from your probiotics, proper handling is essential. It's not just about initial storage but also how you use them daily.

  • Keep probiotics in their original, sealed packaging until use.
  • Avoid leaving probiotics out in the open or transferring them to a pill organizer, which can expose them to light and humidity.
  • If traveling with refrigerated probiotics, use a cooler to maintain a consistent cold temperature.
  • When taking probiotics, avoid consuming them with extremely hot beverages, as this can kill the bacteria.

Shelf-Stable vs. Refrigerated Probiotics: A Comparison

Feature Refrigerated Probiotics Shelf-Stable Probiotics
Storage Requirement Must be refrigerated (2°C-8°C) Stored at room temperature (often below 25°C)
Processing Minimally processed to keep cultures live Freeze-dried (lyophilized) to induce dormancy
Strains Often more delicate strains (e.g., specific Lactobacillus and Bifidobacterium) Often more robust strains or spore-forming bacteria (e.g., Bacillus genus)
Viability Viability maintained through cold chain; can degrade quickly at higher temps Viability maintained through protective coating and dormant state
Convenience Less convenient for travel; requires consistent refrigeration Highly convenient for travel and daily use; no refrigeration needed
Examples Yogurts, kefirs, many traditional supplement capsules Gummies, powders, some advanced capsule formulas

Conclusion: The Nuance of Probiotic Storage

In conclusion, the simple answer to "are probiotics temperature sensitive?" is a resounding yes. However, the more complex truth reveals that this sensitivity varies significantly depending on the specific probiotic strains and the manufacturing process. While many traditional probiotics require consistent refrigeration to remain viable, modern shelf-stable options utilize advanced technologies like freeze-drying to protect the beneficial bacteria from heat and moisture. For consumers, this means that reading and following the label's storage instructions is the single most important step to ensure the product's effectiveness. By understanding these differences, you can make an informed choice and properly store your probiotics to maximize their benefits for your health.

Visit the International Probiotics Association for more information on probiotic storage and handling practices

Frequently Asked Questions

No, not all probiotics need to be refrigerated. Many modern products are freeze-dried and utilize advanced packaging to be shelf-stable and effective at room temperature.

If refrigerated probiotics are left out, especially in warm conditions, the live bacteria can be exposed to heat and moisture, which can cause them to die off and reduce the product's overall potency.

Yes, high heat can destroy beneficial bacteria. For many strains, temperatures exceeding 120°F (49°C) can cause rapid die-off, compromising the probiotic's efficacy.

You can tell by reading the product label. It will clearly state 'refrigerate' if necessary, or provide instructions for room temperature storage (e.g., 'store below 25°C') for shelf-stable versions.

The best way to store probiotics is to follow the manufacturer's specific instructions on the label. This typically means either keeping them refrigerated or in a cool, dry place away from direct sunlight.

It is generally not recommended to take probiotics with hot beverages like tea or coffee, as the high temperature can kill the live bacteria and reduce their effectiveness.

Yes, probiotics in gummies can still be effective. Many gummy formulations use shelf-stable, freeze-dried probiotic strains or specialized packaging to ensure the bacteria remain viable without refrigeration.

References

  1. 1
  2. 2
  3. 3
  4. 4
  5. 5

Medical Disclaimer

This content is for informational purposes only and should not replace professional medical advice.