The process of making cheese involves coagulating milk proteins to separate the solids (curds) from the liquid (whey). Historically, this was achieved using rennet sourced from the stomach lining of young calves. However, for decades, cheesemakers have developed and utilized alternative coagulants, making it possible to find a wide array of vegetarian-friendly cheese in the modern marketplace. Understanding the different types of rennet is key to making informed dietary choices.
Types of Rennet and Their Sources
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Animal Rennet: This traditional coagulant is extracted from the stomach lining of young ruminant animals, such as calves, lambs, or kids, that are still on a milk diet. It primarily contains the enzyme chymosin, which is highly efficient at clotting milk. The flavor profile is often described as complex and is sometimes preferred for certain long-aged, traditional cheeses. Cheeses made with animal rennet are not suitable for vegetarians. 
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Microbial Rennet: A cost-effective and vegetarian alternative, microbial rennet is derived from the fermentation of specific fungi, such as Rhizomucor miehei. While highly effective for coagulation, it was historically associated with some bitterness in long-aged cheeses, though modern techniques have minimized this issue. It is widely used in commercial cheese production today. 
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Fermentation-Produced Chymosin (FPC): This is the most common type of non-animal rennet used globally in commercial cheese production, accounting for over 90% of cheese in some regions. FPC is created by extracting the chymosin-producing gene from a calf's stomach cell and inserting it into a microorganism, like yeast or bacteria, which then produces chymosin during fermentation. The resulting enzyme is identical to the one found in animal rennet but is purified, so the final product contains no animal material or GMOs. Some vegetarians may still consider its origin ethically ambiguous. 
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Vegetable Rennet: Derived from plants with coagulating properties, such as cardoon thistle, figs, or nettles, this type of rennet is less common in mass production due to inconsistent results and a tendency to impart a bitter taste to aged cheeses. It is still used for certain traditional artisanal cheeses, particularly in Mediterranean regions. 
How to Identify Vegetarian-Friendly Cheese
For most cheeses purchased from a standard grocery store, especially those produced in the United States, it is safe to assume a non-animal rennet was used. However, confirming the source of rennet is crucial for strict vegetarians or those with religious dietary requirements. Look for specific labeling clues:
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Words on the label: Search for phrases like “microbial enzymes,” “vegetable rennet,” or “non-animal rennet” in the ingredients list. The simple term “enzymes” without a qualifying description may not be definitive. 
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Certifications: A product may display a 'V' logo or be certified as kosher, which guarantees that no animal-derived rennet was used. 
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Certain cheese types: Be wary of traditional European cheeses that have a protected designation of origin (PDO), such as Parmigiano Reggiano or Gruyère. By law, these authentic cheeses must be made with animal rennet. If you want a vegetarian version, look for one labeled as simply “Parmesan” or “Italian Hard Cheese,” which will use a vegetarian alternative. 
Comparing Rennet Types for Cheese Production
| Feature | Animal Rennet | Microbial Rennet | Vegetable Rennet | 
|---|---|---|---|
| Source | Calf, lamb, or kid stomach lining | Fungi or mold cultures | Thistle, fig, nettles | 
| Availability | More limited supply, higher cost | Widely available, lower cost | Limited and artisanal | 
| Suitability for Vegetarians | No | Yes | Yes | 
| Flavor Impact | Complex, traditional flavor | Generally neutral; can add bitterness to aged cheeses | Variable; can impart bitter notes | 
| Texture | Firmer, more elastic curd | Consistent, generally similar to animal rennet | Can produce a softer curd; inconsistent results | 
| Aging Performance | Stable for long aging periods | Can perform differently during long aging | Not ideal for long aging due to potential bitterness | 
Cheeses Commonly Made Without Animal Rennet
Many fresh and soft cheeses do not use rennet at all, relying instead on acid coagulation, making them inherently vegetarian. Examples include:
- Ricotta
- Paneer
- Cottage Cheese
- Cream Cheese
- Mascarpone
For hard and semi-hard cheeses, many popular North American brands, such as Tillamook, Cabot, and Sargento, use microbial enzymes in their mass-produced products. For imported or artisan cheese, checking the label or asking a cheesemonger is the best approach. Some artisan brands, such as Cypress Grove, also explicitly state their use of vegetarian rennet.
Conclusion: Making the Right Choice for Your Diet
The notion that all cheeses contain animal rennet is a misconception. With the rise of microbial and plant-based alternatives, consumers now have a plethora of delicious and ethical choices available. Whether you are a strict vegetarian or simply curious about food production, the best practice is to always check the ingredient label or, for artisanal and imported products, consult with a knowledgeable seller. The variety of cheeses made with non-animal rennet proves that you can enjoy this versatile food without compromising your dietary principles. For a deeper dive into the science of rennet and cheesemaking, the Wisconsin Cheese website offers valuable resources.